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#yesterday I managed to extract Lutein of green vegetable fatty acids. Therefore I used 10% KOH in methanol for saponification in the first step. Then it was shaked with NaCl and Petrolether (+ 0.1 % BHT as antioxidant) in a separating funnel. At the end it was washed a few times with water dest. and the solvent was evaporated (rotavapor). The yellow phase contains Lutein.. all the other stuff nobody wants is in the green phase . Today I analyzed it via HPLC (Agilent, C18 column, 0.6 ml/min flow, 3 mykroL injection volume, 40-100% methanol gradient, 447 nm wavelength), and got some nice peaks.. tomorrow the results will be discussed in detail (the baseline is not straight)⚕️✔️
#organic #chemistry #methanol #analytics #funnel #yellow #green #laboratory #biotechnology #food #photooftheday #instagood #live #fun #love #thursday #biochemistry #hplc #pottassium #science #research #colorful #summer #shake #it #peak #agilent #mess #university
How to change the fittings and flexible metal ferrules on your GC column? Easy! Take a couple of wrenches, a bit of enthusiasm, steady hands and patience. .
These work sooo much better compared to the glass fitting we used before! Dice we often switch the injectors and columns, constantly cutting the ends of a columns is simply unfeasible. Implementing these metal connectors saves a lot it fine and enhances the column lifetime .
#massspectrometry #gcms #analyticalchemistry #forensicscience #forensicchemistry #chemistry #laboratory #scienceexperiment #womeninscience #nailart #agilent