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Protein over-expression is underway!
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Here I have grown bacterial cultures in these big flasks and induced them to start producing my protein of interest. Thanks little guys!
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I'll now be able to 'harvest' my protein by spinning these bacteria cell in a centrifuge This will give me a concentrated cell pellet which I can purify out just the protein I'm interested in and then put it to the test in the lab ⚗️⚛️
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The rest of the clay dried out before I could finish the tablet! In the last few lines that I was able to write of this section of the Gilgamesh epic, Utanapishti (the Babylonian Noah) tells Gilgamesh how he was given eternal life after surviving the flood. The god Enlil "brought out my wife and made her kneel next to me, he touched our foreheads and stood between us to bless us: 'In the past, Utanapishti was human, but now, Utanapishti and his wife shall become like us gods..."
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#TechniqueTuesday today is about serial dilutions. .
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A lot of people have asked me to explain serial dilutions. This is not necessarily a “technique” but it is something we do in the lab often for many reasons, so here we go!
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A serial dilution is just a way to dilute your sample in a stepwise manner where (usually) the dilution factor is consistent at each step. .
Swipe to see an example of a two fold dilution as a cartoon!
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We often do serial dilutions in the lab when we want to test a range of concentrations for our experiment or even to reduce the number of cells in an assay by a specific and constant amount each time. .
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Serial dilutions are done by all kinds of labs for all different methods and techniques - it’s likely most wet lab scientists have had to do a serial dilution as some stage in their lab life.
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If a serial dilution is something that stresses you out you can also use an online calculator and make sure you plan it out before you start! .
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Happy diluting!
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