Список из самых популярных хештегов по теме #GENOME

If you have problems to amplify a fragment of interest and already checked your primers twice, it might be that you are dealing with a complicated sequence or DNA region. Secondary structures or GC reach regions are more difficult to amplify, so you can try to use these additives to make your PCR work! - - Betaine and Glycerol improves the amplification of DNA by reducing the formation of secondary structures and is often added in commercial kits. Use Betaine or Betaine mono-hydrate, NOT Betaine HCl, at a final concentration of 1.0-1.7M. It can also help by by eliminating the base pair composition dependence of DNA melting. - - DMSO is known for reducing DNA secondary structures, and is often recommended when amplifying GC rich templates. However, DMSO can also greatly reduce the activity of Taq polymerase. So use it in low concentrations. - - 7-deaza-2′-deoxyguanosine is a dGTP analogue that is especially useful for extremely GC rich templates. Success is reported with up to 83% GC. - - Formamide is a widely used organic PCR additives. Formamide is thought to work by binding in the major and minor grooves of DNA, destabilizing the template double-helix and lower melting temperature. - - We found this cool overview of additives in the BiteSizeBio website! They have very cool tips for the lab.

Some psychologists, most famously Carl Jung, have theorised that we're born with the memories and experiences of our ancestors imprinted on our DNA. We're not necessarily unlocking them, but it's possible that our most basic survival instincts might stem from some long ago trauma experienced by a dead relative. ▫️ When your ancestor goes through a period of excessive stress, this “experience” can be added to your genome. An extra layer of information is placed on top of your DNA sequences. The DNA sequence itself doesn’t change, but its “clothes” do, so to speak. Generally speaking, this is known as the vaguely defined field of epigenetics, which means “outside genetics”. ▫️ The authors of a new Science paper describe it more precisely as “transgenerational transmission of environmental information.” It has already been seen in humans – Holocaust survivors’ descendants, for example, have lower levels of the stress-hormone cortisol in their blood, which means they’re more vulnerable to stress and fear. ▫️ This means we can be aware of things we haven’t experienced ourselves and be affected by fears and experiences that are not even our own. ▫️ #memories #inherited #genes #dna #genome #quantumfield #spiritualthoughts #healing #generationaltrauma #ancestralhealing #connected #positivepsychology #univerallove #meditation #breathwork #loveoverfear #ancientwisdom #hiddenknowledge #yoga #soundhealing #psychologyfacts #spiritualscience #spiritualawakening #energyfield #flow #matrix #lawofattraction #earthmedicine #epigenetics #healingfacts

What was the craziest question you were asked by your student? Comment and share your funny experience. Do you know already the Lab.Hacks APP? It’s FOR FREE and assist you in several tasks such as calculations, cell counting, conversions, tips trick tutorials and a lot of fun stuff. DOWNLOAD NOW! It’s FOR FREE! We placed a DOWNLOAD link in our Instagram BIO . #labdibiologia #molecularcellbiology #phd #phdcomics #sciencefun #laboratory #chemistrylab #laboratorio #labhacks#biostudent #sciencelover #labwork #genetics #genetica #genome #biologiamolecular #Sequencing #labtechnician #PhDAdvice #PhDLife#labday #biotechnologist #biologystudent #bioscience #instafacts #instascience # #molbio #massspectronomy

#RNA @thebiotechguy For daily Science updates #Lab #science #scientist #biotech #biotechnology #science #research #biology #biologist #genetic #genomics #gene #genetics #genome #biotechnologist #microbiology #labsofinstagram #labsofinsta #molecularbiology #sciencejokes #biologyjokes #DNA #microbiologia #microbiologist #biostudent #biologystudent #biologystudents #pcr #lablife

How do you solve unruly cell pellets…?. Are you interested in the Lab.Hacks APP? It’s FOR FREE and assist you in several tasks such as calculations, cell counting, conversions, tips trick tutorials and a lot of fun stuff. DOWNLOAD NOW! It’s FOR FREE! We placed a DOWNLOAD link in our Instagram BIO . #labdibiologia #molecularcellbiology #phd #phdcomics #sciencefun #laboratory #chemistrylab #laboratorio #labhacks#biostudent #sciencelover #labwork #genetics #genetica #genome #biologiamolecular #Sequencing #labtechnician #PhDAdvice #PhDLife#labday #biotechnologist #biologystudent #bioscience #instafacts #instascience # #molbio #massspectronomy

When you ressuspend your cells/tissues in Trizol for RNA extraction but still would like to keep the proteins, you can try this protein extraction protocol. After adding chlorophorm to you Trizol suspension and centrifuging, you end up with 3 phases. An aqueous phase containing the RNA, an interphase containing DNA and the organic phase where the proteins are concentrated. You can transfer the RNA to a new tube and add Ethanol to precipitate your DNA and transfer to a new tube. Next, you can follow the illustrated steps to extract protein form the organic phase. Be careful with the Trizol since is extremely toxic. 1. After vortexing your isopropanol-protein phase, incubate for 10min at RT. 2. Perform 2 washing steps: add 500µl 95% EtOH and invert the tube or tap the pellet to really wash it, but without dissolving. Centrifuge at 7600xg for 5mins at 4°C. Discard the supernatant and repeat the wash with 250µl of 95% EtOH. Discard the supernatant and let pellets dry. Inverting the tube in a paper towel helps in the process. 3. Ressuspend the protein: after the pellet is dry, ressuspend it in lysis buffer. This protocol was published by Kopec and collaborators in 2017 and the authors also tested some different SDS concentrations for the lysis buffer. In the end, they came up with the following receipt: EDTA 20mM, NaCl 140mM and 5% SDS. You can easily prepare it from your stock solutions using the Lab.Hacks app! 4. After ressuspending the protein, heat it at 50°C for at least 1h. In the paper, the authors described that up to 18h incubation did not affect protein integrity and phosphorylation. Done! For details check: Kopec AM, Rivera PD, Lacagnina MJ, Hanamsagar R, Bilbo SD. Optimized solubilization of TRIzol-precipitated protein permits Western blotting analysis to maximize data available from brain tissue. J Neurosci Methods. 2017;280:64–76. #labdibiologia #molecularcellbiology #phd #laboratory #chemistrylab  #laboratorio #labhacks #biostudent  #sciencelover #labwork #genetics  #genetica #genome #biologiamolecular #labtechnician #PhDAdvice  #PhDLife #labday #biotechnologist #biologystudent #bioscience #instafacts  #instascience

Sometimes when we need to load our proteins in a SDS-PAGE gel we realize that the protein is to diluted. When working with weak antibodies this can be a real problem.. Cryoconcentration is traditionally used to produce ice cider by freezing out apple juice. The ice moves to the top of the barrel, while the high-sugar juice remains at the bottom and may be fermented. A similar process takes place during freeze-centrifugation of proteins. Cryoconcentrator devices can be really expensive. So why don't you try this DIY method to concentrate your protein? This method was reported in 2012, by a Mexican group for large amount of proteins using falcon tubes and in 2013 they adapted for small amounts of protein prior to SDS-Page (Virgen-Ortiz et al., Anal. Biochem. ePub. 09 Sep 2013). The Mexican team tested their freezecentrifugation technique on four commercial proteins, namely horseradish peroxidase, soybean trypsin inhibitor, horse myoglobin and fructosyltransferase. According to the authors, the cryo-concentrated proteins are easily visible on the gel, in contrast to the diluted samples. Since this method is really interesting, cheap and fast we wanted to share it with you guys! Make sure you test it in some general samples and see if it works for your protein!! We found this cool hack in labtimes.org , a great website with lots of tips for the lab! #labdibiologia#molecularcellbiology #phd#phdcomics #sciencefun #laboratory#chemistrylab #laboratorio #labhacks#biostudent #sciencelover #labwork#genetics #genetica #genome#biologiamolecular #Sequencing#labtechnician #PhDAdvice #PhDLife#labday #biotechnologist#biologystudent #bioscience#instafacts #instascience # #molbio#massspectronomy

Are you interested in the Lab.Hacks APP? It assist you in several tasks such as calculations, cell counting, conversions, tips trick tutorials and a lot of fun stuff. DOWNLOAD NOW! ITS FOR FREE! We placed a DOWNLOAD link in our Instagram BIO . DOWNLOAD NOW!. The upcoming weeks we will show you how to IP your protein and perform Co-IPs and iCLIP experiments. Why would you IP your protein?. For example, to identify proteins which interact with your protein of interest (interactome) or to find DNA or RNA sequences your proteins is binding to (Chip-Seq, Clip-Seq). These techniques are mostly based on an affinity purification with the use of an antibody against your protein of interest -a so called immunoprecipitation or short IP-. Learn in the next posts, which beads and antibodies to use, how the beads should be coated and find a detailed tutorial to finally perform an IP yourself! Have fun! Credits to @martin_of_madness for his beautiful “particles 02” video. He has great animations on his insta! Check it out!. #labdibiologia #molecularcellbiology #phd #phdcomics #sciencefun #laboratory #chemistrylab #laboratorio #labhacks #biostudent #sciencelover #labwork #genetics #genetica #genome #agarose #biologiamolecular #polymerasechainreaction #PCR #qPCR #Sequencing #cDNA #RTPCR #polymerase #labtechnician #PhDAdvice #PhDLife #labday

Cover illustration for @johnshopkinssph magazine about why some people are more susceptible to infectious diseases. The reason may lie in their genome. Thanks to AD Robert Ollinger and Patrick Kirchner at Dog Ear Consultants! #illustration #conceptualillustration #conceptualart #draw #drawing #editorial #magazine #cover #science #dna #genome #epidemiology #disease #art #creative #creativity #davidebonazzi @salzmanart